This arrangement of sepharose affords the use of a variety of different chromatography. Samples must be clear and free from particulate matter, particularly when worki. Nov 15, 2019 preparative routine purification hitrap hp 1 ml and 5 ml iex columns hitrap sp hp and hitrap q hp columns are for routine high resolution, smallscale preparative protein purification. After choosing the optimal medium, prepacked columns and bulk media are available for larger scale preparative work. Available in 10 or 50 ml superflow flasks or hitrap readytouse columns hitrap columns can be operated with a syringe, peristaltic pump, or chromatography system each hitrap column is equiped with one 116 male luer and one 116 female luer. Use hitrap ion exchange and hitrap hic test kits for media screening and simple method. E14 his buffer kit e15 gst buffer kit e16 prescission protease e17 thrombin e18 factor xa e19 vivaspin e20 mini dialysis kit f1 histrap f2 gstrap f3 mbptrap hp f4 streptrap hp f5 hitrap streptavidin hp f hitrap desalting g1 hisprep ff 1610 g2 gstprep ff 1610 g3 hiprep 2610 desalting product index for complete product index see page 23. Cytiva formerly ge healthcare life sciences hitrap capto. Akta start preparative chromatography system proteins. Cytiva hitrap capto iex selection kit designed for use with syringe, peristalic pump, or chromatography systems such as akta design for convenient media screening and optimization studies manufacturer. Hitrap iex selection kit is used for screening of the optimal cytiva ion exchange resin for specific application and development work. Simply enter the quantity you want to purchase and the current availability will appear below the item. Hitrap capto iex selection kit offers a fast, simple, and convenient way for selecting the best ion exchange chromatography media for a given application. Book depository books with free delivery worldwide.
The kit is excellent for screening of method parameters such as selectivity, binding and elution conditions, as well as for small scale purifications. Selection kit for iex resins in hitrap columns cytiva. Hitrap hic selection kit consists of seven 1 ml columns prepacked with hydrophobic interaction chromatography media with different characteristics. Media and column selection refer to ion exchange selection guide code no. The kit is excellent for screening of method parameters such as selectivity, binding and. We dont know when or if this item will be back in stock. Cytivahitrap capto iex selection kit hitrap capto iex. Fisher scientific is the uks leading supplier of laboratory equipment, chemicals, and services used in scientific research, safety, healthcare, and education. Hitrap sp hp, 1 ml and 5 ml hitrap q hp, 1 ml and 5 ml. Instructions 71502768 af histrap affinity columns ge healthcare histrap hp, 1 ml and 5 ml histrap hp is a ready to use column, prepacked with precharged ni sepharose high performance. Hitrap iex selection kit 17600233 hitrap q hp 17115401 hitrap butyl ff 175701 mono s 550gl 17516801 mono q 4. Media selection hitrap tm iex selection kit, including seven different iex media, is used for fast screening of iex ligands and for method optimization. Hitrap columns can be operated with a syringe, peristaltic pump, or chromatography system.
For use with application designed for use with syringe, peristaltic pump and other chromatography systems such as aktadesign. Seven prepacked hitrap columns, each containing a different sepharose ion exchange resin medium. General instructions for affinity purification with hitrap columns. Introduction to purification strategy and purification optimization. Pdf q sepharose high performance and sp sepharose high. Affinity imac pulldown hic iex polishing step d1 d2 e14 a3 a4 a5 a7 b6 b7 b8 b9. Since each of these hbocs require purified stromafree hb as the starting material. Ge life sciences 28934388 hitrap iex selection kit. Hitrapconvenient protein purification fisher scientific. Hitrap iex selection kit consists of seven hitrap columns, prepacked with different sepharose fast flow ion exchange chromatography media for screening of the most appropriate media and conditions to use for specific application and development work.
Formulations are packaged in multiple sizes, and edtafree versions are. Microsoft powerpoint purifier instructional material. Jun, 2008 bicinchoninic acid bca protein assay kit was from pierce ettenleur, the netherlands. Jul 02, 2018 select an anion exchanger q, deae, anx with a buffer ph above pi select a cation exchanger s, sp, cm with a buffer ph below pi start by using a strong anion exchanger q use iex selection kits for screening of the most appropriate ion exchanger if pi of your protein is known if pi of your protein is unknown ka809010718pp. Sample preparation samples should be at the same ph and ionic strength as the starting buffer, and free from. Hitrap iex selection kit consists of seven hitrap 1 ml columns, prepacked with different ion exchangers. Hydrophobic ligands that vary according to their degree of hydrophobicity are available. Hitrap iex selection kit 17600233 hitrap capto iex selection kit 28934388 more on page 10. Screen for the most appropriate hic media and conditions to use for specific application and development work.
Hitrap capto iex selection kit is designed for screening the optimal capto ion exchange or cytiva multimodal chromatography resin prior to scaleup. Recombinant fab fragment, media screening hitrap iex selection kit sample. Hitrap iex selection kits contain sepharose fast flow and sepharose xl in. All columns were from ge healthcare uppsala, sweden. Selection and combination of purification techniques. This prepacked column is ideal for preparative purification. Hitrap columns such as those in the hitrap iex selection kit to quickly and efficiently. Capto offers highvolume throughput with high capacity for fast and efficient purification of large amounts of protein, the company said in a statement. Iex is a binding technique, independent of sample volume. This alliance encroached on the routes used by the ndwandwe alliance, who occupied the region in the north, near the pongola river. Hitrap iex selection kit cytiva, 17600233 sigmaaldrich. Hitrap sp hp and hitrap q hp, 1 and 5 ml columns are listed in table 2. Q sepharose fast flow and sp sepharose fast flow are also available in the.
Protein preparation handbook thermo fisher scientific. Q sepharose fast flow and sp sepharose fast flow are also available in the prefilled predictor 96well filter plates for fast and easy process development. Please read these instructions carefully before using hitrap columns. Hitrap iex selection kit from ge healthcare biocompare. Hitrap capto s, 1 ml hitrap capto deae, 1 ml hitrap capto adhere, 1 ml hitrap capto mmc, 1 ml hitrap capto iex selection kit includes five hitrap 1 ml columns prepacked with different bioprocess chromatography media. Iex separates proteins with differences in surface charge.
Whatman grade gff binder free borosilicate glass microfiber filter used for dna purification, protein precipitation, and landfill leachate toxicity testing. Human plasma high throughput and scaleup automated purification load sample wash elute collect product equilibrate column elute. Five prepacked hitrap columns, each containing a different iex resin, including multimodal ion exchangers. Dec 14, 2017 d9ef92e1f7 difference between auditing and investigation pdf downloadwimpy kid long haul pdf free downloadplan estrategico empresarial pdf downloadhaynes karting manual pdf free downloadiso 485 pdf free downloadluther bibel 1984 pdf downloaddoutrina secrets blavatsky pdf downloaddownload pdf from iphone safari cleaningbusiness ideas in. Hitrap hic selection kit cytiva, 28411007, pack of 7. A hitrap iex selection kit was purchased from ge healthcare. Hitrap 1 ml columns are also included in hitrap iex selection kit together with sp sepharose xl and q sepharose xl. Samples must be clear and free from particulate matter, particularly when working with. Ion exchange chromatography columns for protein purification.
Cytiva formerly ge healthcare life sciences hitrap capto iex selection kit designed for use with syringe, peristalic pump, or chromatography systems such as akta design for convenient media screening and optimization studies. With the purchase of capto viralq the customer is granted a free limited license under. Hitraptm iex selection kit, including seven different iex media, is used for fast. Ge, ge monogram, akta, aktapilot, aktaprocess, amersham, axichrom, biacore, bioprocess, capto, cy, ecl, ecl plex, ecl select, excelgel.
Good chemical stability allows the use of effective cleaningin. Did you know you can view a products availability right on the product page. This selection guide helps you find the appropriate methods and products to obtain proteins. Chemical stability good chemical stability allows the use of effective cleaninginplace cip schemes that result in high recoveries. Cytivahitrap iex selection kit includes seven 1ml iex. Data file 18117288 ab ion exhange chromatography q. Cytiva hitrap iex selection kit includes seven 1ml iex columns. Click on the chromatography method you are interested in to jump to the corresponding selection guide. Hitrap iex selection kit is a tool which offers a fast, easy and convenient way to decide which ion exchange ligand is best for a given application. Protein purification protein production and purification. Hitrap iex columns are ideal for small scale sample clean up. Increasing hydrophobicity butyls ether isopropyl butyl octyl phenyl highly hydrophobic proteins bind tightly to highly hydrophobic ligands.
Select steepest gradient to give acceptable resolution at selected ph. Hitrap iex selection kit consists of seven hitrap 1 ml columns, prepacked. Hiprep sp ff 1610, hiprep cm ff 1610, hiprep q ff 1610. Hitrap iex selection kit is used for screening of the optimal ge ion exchange resin for specific application and development work. Hiprep 1610 columns for ion exchange iex chromatography. Peristaltic flangelessm6 female pump p1 to bottom of hitrap column 1 tubing connector connection of tubing e. Ion exchange chromatography sepharose fast flow ion. Ge life sciences 28934388 hitrap iex selection kit currently unavailable. Prepacked columns for akta start and aktaprime plus systems. For more information about the kit, request data file hitrap iex selection kit, code no. Hitrap iex test kit 17600101 hitrap hic test kit 174901 resource hic test kit 17118701 if a purification is not intended for scale up i. As stated on ge healthcares website, the kit allows fast and easy selection with seven different ionexchange ligands on sepharose fast flow and sepharose xl, prepacked in 1 ml hitrap columns. Iex is a simple and costeffective technique that is also made use of in lv puri.
Hitrap sp hp and hitrap q hp columns characteristics 1 water at room temperature 2 the ranges given are estimates based on our knowledge and experience. Human plasma high throughput and scaleup automated purification load sample wash elute collect product equilibrate. Hitrap capto ion exchange selection kit cytiva, formerly. Available in 10 or 50 ml superflow flasks or hitrap readytouse columns. Cytiva formerly ge healthcare life sciences hitrap. Ribonuclease a, human apotransferrin, lactalbumin, media selection hitrap benzamidine ff high sub sample. Ge healthcare last week launched the hitrap capto iex selection kit, a set of prepacked hitrap 1ml columns for selecting the best capto ion exchange chromatography media for a given application. The five hitrap 1 ml columns are also included in hitrap iex selection kit together with sp sepharose xl and q sepharose xl. Systems where nakedplasmid dna is transferred into the. Select the ion exchange medium according to the objective.
Hitrap capto s, 1 ml hitrap capto deae, 1 ml hitrap capto adhere, 1 ml hitrap capto mmc, 1 ml instructions for use hitrap capto iex selection kit includes five hitrap 1 ml columns prepacked with different bioprocess chromatography media. Purification of bovine hemoglobin via fast performance liquid. Excellent for scaleup from hitrap iex column family. Use hitrap iex selection kit for media scouting and method optimisation. Hitrap capto ion exchange selection kit cytiva, formerly ge.
Pdf protein purification handbook back to collection. Capto q, capto s, capto deae, capto adhere and capto mmc. Manual media selection, method development and optimization. Ion exchange chromatography sepharose fast flow ion exchange. E20 mini dialysis kit f hitrap desalting f15 hitrap protein a f16 hitrap protein g. Free essays, homework help, flashcards, research papers, book reports, term. After choosing the optimal medium, prepacked columns and bulk media. The seven columns included in the kit are also available as individual hitrap 1ml and 5ml columns.
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